Affiliated to Rajasthan Technical University, Kota
Approved by AICTE, New Delhi
99291440761800-123-7701 (Toll Free)



Value Added Certificate Cum Training Programmes

Eligibility: 12th pass or Graduate

For Registration & further information please contact: Modi Educational Complex, Dadabari Ext. , Kota-324009

Tel.- +91-0744-2505421, Tollfree- 1800-123-7701.

Separate hostel facilities for girls & boys. Hostel fee starts from 6500/- included breakfast, lunch & dinner (A.C. & Cooler rooms)

Advanced Biology

 

Module III : Molecular Biology and Immunological Techniques (Duration - 15 days /30 days/45 days )

 

 

 

 

 

 

 

Molecular Biology and Immunological Techniques !

  • Working in Molecular Biology Laboratory. Preparation of Reagents, Stock Solutions & Methods of Labeling and Storage.
  • General and Safety Instructions.
  • Extraction of Genomic DNA from Plants material.
  • To Perform Isolation of genomic DNA using CTAB method.
  • Purification of extracted plant genomic DNA material.
  • Electrophoresis of Genomic DNA.
  • Alginate for cell immobilization
  • Extraction of Plasmid DNA from E. coli
  • Purification of the extracted Plasmid DNA.
  • Electrophoresis of purified Plasmid DNA.
  • Perform the technique of sucrose centrifugation to isolate sub cellular fraction and identify it by using Janus green.
  • Introduction of SDS page electrophoresis.
  • Perform an experiment to isolate the Genetic material from the given animal tissue (Goat  liver).
  • To perform & analyze the technique of Ouchterlony Double Diffusion.
  • To test the antigen sample by sandwich “Dot Elisa” method
  • Restriction Digestion of DNA by Restriction Endonucleases & Separation of Digestion Fragments by Electrophoresis.
  • To perform & analyze the technique of Rocket immune electrophoresis
  • To perform & analyze the technique of Radial immune diffusion.